Labortory Guide in Bacteriology (1905) by Paul Gustav Heinemann

Labortory Guide in Bacteriology (1905)

Paul Gustav Heinemann
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  • ISBN
    9781459018099 / 1459018095
  • Title Labortory Guide in Bacteriology (1905)
  • Author Paul Gustav Heinemann
  • Format
  • Year 2010
  • Pages 28
  • Publisher
    General Books
  • Imprint General Books
  • Language English
  • Dimensions 229mm x 6mm x 152mm


This is an OCR edition without illustrations or index. It may have numerous typos or missing text. However, purchasers can download a free scanned copy of the original rare book from GeneralBooksClub.com. You can also preview excerpts from the book there. Purchasers are also entitled to a free trial membership in the General Books Club where they can select from more than a million books without charge. Original Published by: The University of Chicago press in 1905 in 298 pages; Subjects: Bacteriology; Juvenile Nonfiction / Science & Nature / General; Medical / Microbiology; Science / General; Science / Life Sciences / Bacteriology; Science / History; Science / Life Sciences / Biology / Microbiology; Science / Philosophy & Social Aspects;

Publisher Description

This historic book may have numerous typos and missing text. Purchasers can download a free scanned copy of the original book (without typos) from the publisher. Not indexed. Not illustrated. 1905. Excerpt: ... CHAPTER II CLEANING, PREPARING, AND STERILIZING GLASSWARE EXERCISE I. CLEANING GLASSWARE Culture-tubes, flasks, fermentation-tubes, and Petri dishes must be free from organic matter, acids, and alkalis. They are best cleaned as follows: a) Completely immerse them in a vessel containing soapsuds or soap-powder, boil for at least 10 minutes, then thoroughly brush them with the tube-brush; or, immerse them for an hour or more in the chromic-acid cleaning-mixture, consisting of—Potassium dichromate 60 parts Water 300 parts Concentrated sulphuric acid 460 parts The sulphuric acid is to be added slowly with constant stirring. b) Rinse thoroughly in tap-water. c) Again use the tube-brush, and soap and water, if necessary. d) Rinse very thoroughly, and get rid of every trace of acid, soap, or alkali. e) Turn upside down in a basket, and heat for 20 minutes in the hot-air sterilizer until perfectly dry. EXERCISE II. PLUGGING OF CULTURE-TUBES Directions for plugging.—Take a small amount of ordinary, non-absorbent cotton of good quality, fold it once, and by means of a glass rod push it into the mouth Fig. 2 Plugged Culture-Tube of the tube to the depth of about of an inch; or roll the cotton into cylinders of the same diameter as the culure-tube and insert it into the mouth (Fig. 2). The plug should allow the interchange of air, and at the same time be tight enough to hold the weight of the tube, and with no crevices on the side. The glass rod should never be pushed through the cotton. EXERCISE III STERILIZATION OF GLASSWARE Sterilization is the process of killing all micro-organisms. This may be accomplished by heat, by certain chemicals, or by filtration. Sterilization by heat may be accomplished—1. By dry heat; this method is applied to sterilization of many...

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Labortory Guide in Bacteriology (1905)