X-ray microanalysis is a widely used technique in studies of cell and tissue elemental composition. It has applications in many different areas of biology, including animal and plant cell physiology, medicine, pathology and studies on environmental pollution. The author presents an up-to-date look at the use of X-ray microanalysis in biology and discusses the potential of the technique for future research. The author tackles four major aspects of X-ray microanalysis - detection and quantification of X-rays, associated techniques, specimen preparation, and applications. The book is clearly written and highly illustrated, with each section containing chapters by experts in the field.
Preface; Part I. Detection and Quantification of X-Rays: 1. Recent developments in X-ray detectors and their relevance in biological X-ray microanalysis C. J. G. Blok-van Hoek; 2. Quantitative software for biological applications of X-ray microanalysis R. B. Shen; 3. X-ray mapping techniques in biology J. Wood; 4. Quantitative X-ray microanalysis of thin sections in biology: appraisal and interpretation of results A. Warley; Part II. Associated Techniques: 5. Proton probe microanalysis in biology: general principles and applications for the study of Alzheimer's disease F. Watt; 6. Electron energy-loss spectroscopy and electron probe X-ray microanalysis of biological material W. C. De Bruijn; Part III. Specimen Preparation: 7. Rapid freezing techniques for biological electron probe microanalysis K. Zierold; 8. Radiation damage and low temperature X-ray microanalysis T. Von Zglinicki; 9. X-ray microanalysis in histochemistry A. T. Sumner; 10. Sprayed microdroplets: methods and applications A. J. Morgan and C. Winters; 11. X-ray microanalysis of biological fluids: applications to investigations in renal physiology N. Roinel and C. De Rouffignac; Part IV. Applications: 12. Electron probe X-ray microanalysis of bacterial cells: general applications and specific studies on plant pathogenic bacteria D. C. Sigee and N. Hodson; 13. Ion localisation in plant cells using the combined techniques of freeze-substitution and X-ray microanalysis M. A. Hajibagheri and T. J. Flowers; 14. Electron probe X-ray microanalysis of diffusible ions in cells and tissues from invertebrate animals B. L. Gupta; 15. X-ray microanalysis in pollution studies J. A. Nott; 16. X-ray microanalysis in biomaterials research H. K. Joeten; 17. Applications of X-ray microanalysis in biomedicine: an overview G. M. Roomans; 18. X-ray microanalysis of cultured mammalian cells J. Wroblewski.
Cambridge University Press
Cambridge University Press
Experimental Techniques and Applications
Place of Publication
Country of Publication
Alice Warley, John Morgan, Adrian T. Sumner, David C. Sigee
Department of Physiology, St Thomas's Hospital, School of Pure and Applied Biology, University of Wales, MRC Human Genetics Unit, Western General Hospital, Edinburgh
109 b/w illus. 46 tables
X-RAY MICROANALYSIS IN BIOLOGY