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Enzyme Kinetics and Mechanism

  • Hardcover
Focuses on steady-state enzyme kinetics. This book covers kinetic mechanism, relative rates of steps along the reaction pathway, and chemical mechanism - including acid-base chemistry and transition state structure. It is intended for enzymologists and molecular biologists.
Enzyme Kinetics and Mechanism
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Enzyme Kinetics and Mechanism is a comprehensive textbook on steady-state enzyme kinetics. Organized according to the experimental process, the text covers kinetic mechanism, relative rates of steps along the reaction pathway, and chemical mechanism--including acid-base chemistry and transition state structure. Practical examples taken from the literature demonstrate theory throughout. The book also features numerous general experimental protocols and how-to explanations for interpreting kinetic data. Written in clear, accessible language, the book will enable graduate students well-versed in biochemistry to understand and describe data at the fundamental level. Enzymologists and molecular biologists will find the text a useful reference.
Paul F. Cook is the Grace B. Kerr Centennial Professor of Chemistry and Biochemistry at the University of Oklahoma. His awards and achievements twice included the Alexander von Humboldt Research Fellowship and he was the recipient of the National Institutes of Health Research Career Development Award from 1983 to 1988. Professor Cook is Associate Editor of Protein and Peptide Letters and Executive Editor of Proteins and Proteomics. W.W. Cleland is the M.J. Johnson Professor and Steenbock Professor of Chemical Science at the Enzyme Institute at University of Wisconsin-Madison. In the early 1960s, ClelandaaC--a s landmark papers created a theoretical framework for the study of steady-state kinetics of multisubstrate enzymes and laid the foundation for all subsequent steady-state studies. He serves on the editorial boards of Journal of Biological Chemistry and of Biochemistry. Recognized by many as the aaC--A"father of modern enzyme kinetics,aaC--Au Professor Cleland was elected to the National Academy of Sciences in 1985.
1. Nomenclature 2. Introduction to Kinetics 3. Enzyme Assays 4. Derivation of Initial Velocity Rate Equations and Data Processing 5. Initial Velocity Studies in the Absence of Added Inhibitors 6. Initial Velocity Studies: Presence of Added Inhibitors 7. Pre-Steady State and Relaxation Kinetics 8. Isotopic Probes of Kinetic Mechanism 9. Isotope Effects as a Probe of Mechanism 10. pH Dependence of Kinetic Parameters and Isotope Effects Appendix I. King and Altman Patterns and Distribution Equations Appendix II. Rate Equations, Definitions of Kinetic and Inhibition Constants, Haldanes, Distribution Equations and Rate Constants for a Number of Multireactant Mechanisms
"Enzyme Kinetics and Mechanism is an advanced text with meticulous details...[that] gives 'hands on' explanations to the target readership..." Journal of the Higher Education Academy Physical Sciences Centre, June 2008
Publisher
Garland Publishing
Language
English
ISBN-10
0815341407
ISBN-13
9780815341406
Media
Book
Format
Hardcover
DEWEY
572.744
Illustrations
Yes
Year
2007
Publication Date
2007-03-22
Imprint
Garland Publishing Inc
Place of Publication
CT
Country of Publication
United States
Edition
1st
Author
W.W. Cleland, Paul F. Cook
Short Title
ENZYME KINETICS & MECHANISM
Pages
404
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